“On-line internal surface reversed-phase cleaning”: The direct HPLC analysis of crude biological samples Application to the kinetics of degradation of oligonucleotides in cell culture medium
Identifieur interne : 004790 ( Main/Exploration ); précédent : 004789; suivant : 004791“On-line internal surface reversed-phase cleaning”: The direct HPLC analysis of crude biological samples Application to the kinetics of degradation of oligonucleotides in cell culture medium
Auteurs : Alain Pompon [France] ; Isabelle Lefebvre [France] ; Jean-Louis Imbach [France]Source :
- Biochemical Pharmacology [ 0006-2952 ] ; 1992.
English descriptors
- KwdEn :
- Teeft :
- Absorbance, Ammonium acetate, Anal chem, Analytes, Analytical column, Anticonvulsant drugs, Antisense, Biological fluids, Biological samples, Biological systems, Cell culture, Cell culture media, Cell culture medium, Chem, Chem pharm bull, Chromatogr, Chromatographic conditions, Clin chem, Culture medium, Degradation, Degradation products, Delivery system, Derivated antihiv nucleosides, Direct analysis, Direct hplc analysis, Direct injection, Elution order, Enzymatic stability, Excellent results, Extensive sample preparation, Flow rate, Frontal analysis, Further development, Hplc, Hplc analysis, Human serum, Initial concentration, Internal surface, Internal surface columns, Ionic strength, Isrp, Isrp cleaning, Isrp material, Isrp precolumn, Linear gradient, Liquid chromatography, Mini, Mini absorbance, Online isrp cleaning, Organic modifier, Phosphotriester prodrugs, Potassium chloride, Precolumn, Recherche contre, Sample preparation, Silica, Silica support, Stock solutions, Unpublished data, Uremic serum.
Abstract
Abstract: An “on-line” HPLC analysis of crude biological samples is described. A precolumn of internal surface reversed-phase material allows the passage of proteins and other unwanted products while retaining analytes which are transferred, concentrated and chromatographed on a conventional reverse-phase or ion-exchange HPLC column. This protocol allows precise kinetics of the degradation of an oligonucleotide in cell culture to be obtained without radiolabelling or sample preparation.
Url:
DOI: 10.1016/0006-2952(92)90709-R
Affiliations:
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A precolumn of internal surface reversed-phase material allows the passage of proteins and other unwanted products while retaining analytes which are transferred, concentrated and chromatographed on a conventional reverse-phase or ion-exchange HPLC column. This protocol allows precise kinetics of the degradation of an oligonucleotide in cell culture to be obtained without radiolabelling or sample preparation.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Montpellier</li></settlement><orgName><li>Université Montpellier 2</li></orgName></list><tree><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Pompon, Alain" sort="Pompon, Alain" uniqKey="Pompon A" first="Alain" last="Pompon">Alain Pompon</name></region><name sortKey="Imbach, Jean Louis" sort="Imbach, Jean Louis" uniqKey="Imbach J" first="Jean-Louis" last="Imbach">Jean-Louis Imbach</name><name sortKey="Lefebvre, Isabelle" sort="Lefebvre, Isabelle" uniqKey="Lefebvre I" first="Isabelle" last="Lefebvre">Isabelle Lefebvre</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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